MicroRNA-3137/Nuclear Protein Transcription Regulator 1 Axis Regulates Cervical Cancer Cells Proliferation Apoptosis and Autophagy by PI3K/AKT/mTOR pathway

Cervical cancer belongs to the second most frequent kind of in female as well a major cause mortality all over world. MicroRNAs are crucial modulators multiple diseases. Based on GSE86100 database, microRNA-3137 expression was low cervical tissues and difference significant, thus we further probed potential along with mechanism cells. Reverse transcription-quantitative polymerase chain reaction together Western blot implemented for examining gene expression. 5-ethynyl-2'-deoxyuridine flow cytometry analyses were adopted assessing cells proliferation apoptosis. Immunofluorescence measure fluorescence intensity autophagy marker LC3II. Target scan luciferase reporter assay confirming direct binding nuclear protein transcriptional regulator 1. The results revealed that, under expressed Overexpressed repressed while elevated Besides, 1 directly targeted negatively regulated by highly Moreover, silence promoted rescue assays validated that microRNA-3137/nuclear axis suppressed apoptosis through inactivation phosphatidylinositol-3-kinase/ kinase B/mammalian target rapamycin pathway. Collectively, our study demonstrated affected proliferation, apoptosis, means inhibiting phosphatidylinositol-3-kinase/protein B/ mammalian pathway, which may offer novel insights into progression therapy targeting therapy.